LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
B.Sc. DEGREE EXAMINATION – CHEMISTRY & ADVAN. ZOOL.& BIO-TECHN.
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THIRD SEMESTER – April 2009
PB 3200 – GENERAL MICROBIOLOGY
Date & Time: 16/04/2009 / 1:00 – 4:00 Dept. No. Max. : 100 Marks
PART A
Answer all questions (20 Marks)
- Choose the correct answer (5×1 =5 Marks)
- Which of the following is not true for fungi
a.heterotrophic b. saprophytic c. parasitic d. autotrophic
- Barophilic microorganisms are those which grow at
- a. cold temperature high pressure
- high temperature d. high pH values
- Transcription and translation are related to
- DNA replication b. glycolysis
- protein synthesis d. the electron transport chain
- Which of the following is not true for Mycoplasma?
- pleomorphic b. membrane contains sterol
- penicillin resistant d. non- self reproducing
- Name the enzyme which is a substitute for calf renin
- alkaline protease b. acid protease
- neutral protease d. lipase
II State whether the following statements are true or false (5×1= 5 Marks)
- The phycobiliproteins are characteristic of green algae.
- Bacterial flagella are composed of a carbohydrate known as peptidoglycan.
- The bases adenine, guanine and cytosine are found in both RNA and DNA, but RNA
has thymine also.
- TMV is sap transmissible and enters the host through wounds.
- Malted barley is used in beer production.
III Complete the following (5×1 = 5 Marks)
- The reserve food material in fungi is ____________.
- __________ microorganisms are those capable of living in different
environments.
- The operon has been studied in depth in the common bacterium_________.
- _____________ is grown in highly specified culture medium containing sterol.
- The source for streptomycin is __________.
- Answer all, each in about 50 words (5x 1 = 5 Marks)
- What are capsomeres?
- Write short notes on inclusion bodies.
- What is transformation?
- Suggest any two measures to control tobacco mosaic disease.
- Define sulphate reduction.
PART B
Answer any five, each within 350 words only. Draw diagrams and flowcharts wherever
necessary. (5x 8= 40 Marks)
- Discuss briefly about the general features of viruses.
- Describe the classification of bacteria based on shapes and flagellation.
- Explain the stages of microbial growth curve.
- Describe the process of bacterial conjugation.
- Give a brief account of citrus canker.
- Write about the pathogen, symptoms and control measures of wheat rust disease.
- Give an account of trickling filters.
- Write notes on penicillin production.
PART C
Answer the following, each within 1500 words only. Draw diagrams and flowcharts wherever necessary. (2×20= 40 Marks)
- Explain in detail about the following :
- salient features of algae ii) salient features of fungi
OR
- Write notes on
- i) Nitrogen cycle ii) Microbial enzymes
30 a. Explain the various pure culture techniques and add a note on maintenance of pure cultures.
OR
- Write an essay on DNA replication in E. coli.
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