LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.
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FIFTH SEMESTER – April 2009
PB 5504 – PLANT BIOTECHNOLOGY
Date & Time: 24/04/2009 / 1:00 – 4:00 Dept. No. Max. : 100 Marks
PART – A (20 marks)
- Choose the correct answer: (5 x 1 = 5)
- In plant tissue culture commonly used medium is
- S. medium b) Nitsch medium c) B5 medium d) white’s medium
- Laminar airflow cabinet consists of
- HAPA filter b) HEPA filter c) HEAP filter d) HAPE filter
- Most suitable explant for the production of haploid plant is
- ovule b) anther c) microspore d) stamen
- Cyanobacteria are commonly called as
- Red algae b) Green algae c) Blue algae d) Blue green algae
- The purified form of agar is
- agarose b) Sucrose c) cellulose d) alginate
- State the following statements are true or false: (5 x 1 = 5)
- T-DNA refers to transfer DNA.
- Sodium alginate is used as fusogent.
- Phenosafranin is used to identigy dead protoplast.
- pBR 322 is constructed from PUC.
- Agar-agar is extracted from the algae Gracilaria.
- Complete the following: (5 x 1 = 5)
- Taq DNA polymerase enzyme is extracted from __________________.
- sodium alginate is extracted from _________________.
- Spirulina contains ________________ pigment.
- Knudson medium is used for _______________ culture.
- Single stranded DNA is present in ______________ virus.
- Answer all in about 50 words: (5 x 1 = 5)
- What is an explant?
- Define micropropagation.
- What is single cell protein?
- Mention any two algal seaweeds.
- What is chimeric DNA?
PART – B
Answer any FIVE, each within 350 words. Draw diagrams/ flow charts wherever necessary:
(5 x 8 = 40)
- Describe the Polymerase chain Reaction technology.
- Enumerate the significance of Blue Green Algae.
- Write notes on: a) artificial seed b)restriction enzyme
- Briefly explain the various physical method of sterilization techniques.
- Describe the genetic organization and function of chloroplast genome.
- Give the biotechnological importance of yeast.
- Write notes on phycocolloids.
- Define callus. Describe the types and add a note on its significance.
PART – C
Answer the following each within 1500 words only. Draw diagrams/ flow charts wherever necessary: (2 x 20 = 40)
- a) Mention the significance of tissue culture in plant science
(OR)
- b) Explain the genetic aspects involved in nitrogen fixation in Legume-Rhizobium
interaction.
- a) Give an account of somatic hybridization. Add a note on its significance.
(OR)
- b) What is rDNA technology? Describe any four gene transfer techniques used in rDNA
technology.
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