LOYOLA  COLLEGE (AUTONOMOUS), CHENNAI-600 034.

M.Sc. DEGREE  EXAMINATION  – ZOOLOGY

THIRD SEMESTER  – APRIL 2003

ZO 3802/Z  924  BIOTECHNOLOGY

15.04.2003

1.00 – 4.00                                                                                             Max: 100 Marks

PART – A                                       (10 ´ 2 = 20 Marks)

Answer ALL the questions

 

1. What are vaccines? Give examples.
2. Differentiate primary cell line from established cell line.
3. How do you isolate cells for culturing?
4. Comment on Biodegradation.
5. Expand the following

(i)  WTO  (ii)UNIDO  (iii) IPR+PL     (ii) UNIDO   (iv) GATT

6. Comment on Biosensors.
7. Define fermentation.
8. Differentiate upstream processing from down stream processing.
9. What is a Bioreactor?
10. Comment on ‘Southern Blotting’.

 

 

                                                PART – B                                        (10 ´ 4 = 40 Marks)

Answer any FOUR questions

 

11. Describe what the Hybridoma technology and its uses?
12. Explain the role of biotechnology in Agriculture.
13. What is ‘Molecular cloning’- Explain?
14. Describe the Indian ‘Biotechnology scenario’.
15. Describe the importance of patenting.
16. Briefly describe ethical issues arising out of Biotechnology.
17. Describe the Global ‘Biotechnology scenario’

PART – C                                       (20 ´ 2 = 40 Marks)

Answer any two questions

 

18. Give an account on environmental Biotechnology.
19. Write an essay on the technological uses of Tissue culture.
20. Discuss the recent advancement in health care Biotechnology.
21. Explain the role of Biotechnology in sustainable development.

 

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI  – 600 034

M.Sc. DEGREE EXAMINATION –  ZOOLOGY

THIRD SEMESTERK-APRIL 2004

ZO 3802/ Z  924  BIOTECHNOLOGY

20.04.2004

1.00 –4.00                                                                                         Max. 100 Marks

 

PART  – A                (10 ´2 = 20  Marks)

Answer ALL questions.

 

1. Differentiate primary cell line from established cell line.
2. How do you isolate cells for culturing?
3. Comment on Biodegradation.
4. Write a note on Bio mass production.
5. Expand the following (i) GATT (ii) WIPO (iii) IPR & PL (iv) UNIDO
6. Define fermentation.
7. Comment an ‘Bio sensor’.
8. What are ‘Idiolites’?
9. What is direct cloning?
10. Comment on ‘Partial Digestion’.

 

 

PART – B                              (4 ´ 10 = 40 Marks)

Answer any FOUR questions.

 

11. Describe the Indian Biotechnology scenario.
12. Describe the fermentation process for any three products.
13. Bring out the role of biotechnology in aquaculture.
14. Bring out the role of Hybridoma technology and its uses.
15. Describe the role of micro organisms in pollution control.
16. Describe the process of C-DNA cloning and its uses.

 

 

PART – C                                    (2 ´ 20 = 40 Marks)

Answer any TWO questions.

 

17. Give an account of intellectual property and patent rights.
18. Describe the role of Biotechnology in Agriculture.
19. Discuss what is health care biotechnology and its present status.
20. Give on account an the Bio reactor designing.

 

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – ZOOLOGY

TS 41

FOURTH SEMESTER – APRIL 2007

ZO 4811 – BIOTECHNOLOGY

 

 

 

Date & Time: 20/04/2007 / 9:00 – 12:00          Dept. No.                                                          Max. : 100 Marks

 

 

 

PART – A

 

Answer all the questions:                                                                         10 x 2 = 20

 

1. What is transposons?
2. Comment on bioterrorism.
3. Differentiate Biodegradation and Bioremediation.
4. Comment on tissue specific knockout mice.
5. What are the uses of organic acid?
6. Give the principle of Western blotting.
7. What is transgenic chicken?
8. Comment on adenoviruses.
9. Define cryobiology.
10. What are the key concepts of recombinant DNA technology?

 

PART – B

 

Answer any four of the following                                                             4 x 10 = 40

 

11. Give the details on infra structure requirement for plant tissue culture.
12. Explain the process of Nitrogen fixation.
13. Describe briefly the genomic library.
14. Some achievements are made through transgenic technology in plants and animals– Discuss.
15. Describe: Genetically modified crop and it is a boon or a bane?
16. Explain the usage of DNA fingerprint technique in different fields.

 

 

PART – C

 

Answer any Two of the following                                                             2 x 20 = 40

 

17. Write an essay on human gene therapy and add a note on various dimensions of its positive and negative impacts.
18. Write an essay on the process of in vitro
19. Explain the various aspects of genomic library.
20. Write an essay on cloning and add a note on ethical issues involved.

 

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – ZOOLOGY

DE 37

FOURTH SEMESTER – APRIL 2008

ZO 4811 – BIOTECHNOLOGY

 

 

 

Date : 23/04/2008            Dept. No.                                        Max. : 100 Marks

Time : 9:00 – 12:00

SECTION-A

Answer all the question                                                                  (10 x 2 = 20)

1. What is meant by rDNA technology? Give example.
2. Comment on gene bank.
3. Draw the biotechnology tree.
4. What are the impacts of biotechnology?
5. What are ‘nif’ genes? Give example.
6. Differentiate protoplasmic fusion from cytoplasmic fusion.
7. What is meant hybridoma technology?
8. Differentiate bioleaching from biomining
9. Write a note on ‘Transgenesis’
10. Define Fermination? Give example.

                                         SECTION-B

Answer any Four                                                                              (4 x 10 = 40)

1. Explain the Monoclonal antibodies and its uses.
2. Describe cloning vector of Agrobacterium tumefaciens.
3. Bring out the ethical issues in biotechnology.
4. Describe the role of biotechnology on environmental management.
5. Explain the salt tolerant varieties of species.
6. Schematically represent the basic steps of gene cloning.

SECTION-C      

Answer any Two                                                                               (2 x 20 = 40)

1. Explain in detail patent law and intellectual property right
2. Discuss the global and Indian scenario regarding biotechnology
3. Write an essay on human gene therapy with related diseases
4. Explain a) simian virus 40-gene transfer technology

b)Schematically represent cryopreservation.

 

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – ZOOLOGY

FOURTH SEMESTER – APRIL 2012

ZO 4811 – BIOTECHNOLOGY

 

 

Date : 20-04-2012             Dept. No.                                        Max. : 100 Marks

Time : 1:00 – 4:00

 

SECTION – A

Answer ALL questions:                                                                                                             (10×2= 20)

 

1. Distinguish event specific from construct specific detection of GMO.
2. Name any four restriction enzymes.
3. Sketch PBR 322
4. Comment on golden rice and azolla.
5. Define nutrigenomics.
6. State the significance of knockout mice and suicide gene therapy.
7. Distinguish morphological markers from biochemical markers.
8. What do you know about Integria?
9. Define downstream processing
10. Expand: a) DMSO    b) WIPO

SECTION – B

Answer any FOUR questions  briefly:                                                                                      (4×10= 40)

 

11. How do you create transgenic animals using the provision of microinjection

and embryonic stem cells?

12. Draft a protocol for cryopreservation and add its applications.
13. How do you immobilize enzymes? Mention its significance.
14. State the properties of restriction recognition sites
15. Illustrate direct and cell based delivery system towards gene therapy.
16. What are the challenges in DNA computing?

 

SECTION – C

Answer any TWO questions in detail:                                                                                     (2×20= 40)

 

17. Illustrate nucleic acid hybridization and DNA finger printing techniques.

 

18. Describe any four methods of cloning with vector

 

19. Propose a design of conventional bioreactor with all their components and

explain its implications in bioprocessing.

 

20. Highlight on : a) Risks of IVF  and infertility therapy and
21. b) Risks of Biotechniques.

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – ZOOLOGY

FOURTH SEMESTER – NOVEMBER 2012

ZO 4811 – BIOTECHNOLOGY

 

 

Date : 06/11/2012            Dept. No.                                        Max. : 100 Marks

Time : 1:00 – 4:00

SECTION – A

Answer ALL questions:                                                                                                        (10×2= 20)

 

1. Distinguish bioleaching from bioremediation.
2. Name the filter paper being used in southern blotting and northern blotting.
3. State Wallac rule on melting temperature.
4. Sketch Ti Plasmid.
5. Distinguish monoclonal from polyclonal antibodies.
6. What are molecular markers? Name any two of them.
7. What are base cutters? Mention the types.
8. What are magic bullets? State the importance.
9. What are shuttle vectors? Mention their application.
10. Expand: a) PCB b) HRCS

 

 

SECTION – B

 

Answer any FOUR of the following briefly:                                                                         (4×10= 40)

 

11. How do microbes oxidize Fe₂⁺ into Fe₃⁺ ? Mention its significance?
12. What are the criticisms raised against GMO?
13. “Heart of fermentation is bioreactor” – Substantiate.
14. What are the significant decisions taken by Govt of India regarding patent

system?

15. What are the risks and ethics involved in gene therapy?
16. Describe any two cloning vectors.

 

SECTION – C

 

Answer any TWO of the following in detail:                                                                         (2×20=40)

 

17. How do you resort to animal cell culture and organ culture? What are their applications?
18. What are the strategies and tools involved in DNA computing?
19. Elucidate the strategies involved in large scale production of enzymes.
20. Discuss the impact of biotechnology on Agricultural crops.

 

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