LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

    B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

 

ED 12

FIFTH SEMESTER – November 2008

PB 5406 – IMMUNOLOGY AND IMMUNO TECHNOLOGY

 

 

 

Date : 12-11-08                     Dept. No.                                        Max. : 100 Marks

Time : 9:00 – 12:00

 

PART – A                                          (20 marks)

 

Answer ALL questions

 

1. Choose the correct answer             (5 x 1 = 5)

 

1. The Greek word vacca means
2. a) disease b) immunity     c) small pox                 d) cow
3. C_H has _____ amino acids
4. a) 110 b) 220              c) 100                          d) 330
5. The enzyme used in ELISA is
6. a) protease b) lipase           c) peroxidase               d) amylase
7. Which of the immunoglobin passes from mother into the foetus?
8. a) Ig A b) Ig G                        c) Ig M                                    d) Ig E
9. Which animal is used in large scale production of antisera?
10. a) rabbit             b) guinea pig   c) buffalo                    d) horse

 

 

1. State whether true or false             (5 x 1 = 5)

 

6. Rabies vaccine was invented by Koch.
7. The decreased immunity is also related to hormonal disorders.
8. Haptens are large molecular substances.
9. ELISA uses radiolabelled antibodies.
10. Myeloma cells are used because they are immortal.

 

 

III. Complete the following                                                                                                    (5 x 1  = 5 )

 

11. Tears contain the enzyme _______.
12. The immunoglobin ______ is associated with allergic response.
13. The principle of blood grouping is _______ .
14. ________ stem cells are seen in the Bone marrow.
15. ___________ medium is used to grow hybridoma.

 

1. Answer the following each in about 50 words (5 x 1= 5)

 

16. List any two contribution of Pasteur.
17. What is an epitope?
18. Mention two characteristics of IgE.
19. Define agglutination.
20. What is a Myeloma cell?

 

 

 

PART  B

 

Answer any FIVE of the following, each answer not exceeding 350 words

Draw diagrams and flowcharts wherever necessary.                                                  ( 5 x 8= 40 marks)

 

21. Write notes on the important breakthroughs in immunology.
22. Give an outline on immunity schedule.
23. Write short notes on antigens.
24. Discuss briefly about immunodiffusion.
25. Write short notes on radioimmunoassay.
26. Discuss briefly about the organs of the immune system.
27. Give an account of cytokines and their significance.
28. What are polyclonal antibodies? How are they produced?

 

 

Part C

 

Answer the following, each answer not exceeding 1500 words.

Draw diagrams and flowcharts wherever necessary.                                                  (2 x 20 = 40 marks)

 

29. Explain in detail about vaccines and their types.

 

(or)

 

1. Give an account on antibodies, their properties and structure.

 

30. Write detailed notes on cell mediated immunity.

 

(or)

 

1. Discuss in detail about the steps involved in the production of monoclonal antibodies and list out the applications of MAbs.

 

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     LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

VE 48

FIFTH SEMESTER – April 2009

PB 5406 – IMMUNOLOGY AND IMMUNO TECHNOLOGY

 

 

 

Date & Time: 30/04/2009 / 1:00 – 4:00       Dept. No.                                                       Max. : 100 Marks

 

 

PART A (20 marks)

1. Choose the correct answer (5 x 1 = 5)

 

1. Edward Jenner is associated with vaccines against
2. a) Rabies b) Chicken pox                        c) Small pox                d) Hepatitis
3. Light chain has _____ amino acids
4. a) 110 b) 220              c) 100                          d) 330
5. The enzyme used in ELISA is
6. a) protease b) lipase           c) peroxidase               d) amylase
7. Which of the immunoglobin passes from mother into the foetus?
8. a) Ig A b) Ig G                        c) Ig M                                    d) Ig E
9. Hybridoma technology was invented by
10. a) Kohler b) Hanstein                  c) Pasteur                    d) Lister

 

1. State whether the following statements are true or false (5 x 1 = 5)

 

6. Pasteur invented Rabies vaccine.
7. Ig D is a pentamer.
8. Precipitaion occurs with soluble antigens.
9. Western blotting is used in HIV detection.
10. In Monoclonal antibody production, Myeloma cells are used because they are immortal.

 

III. Complete the following                                                  (5 x 1  = 5 )

 

11. Vaccine is derived from a Greek word which means ______.
12. The immunoglobin IgE is associated with __________.
13. The principle of blood grouping is _______ .
14. The technique that involves transfer of proteins to membrane is ________
15. On activation, the B cells becomes

 

1. Answer the following, each in about 50 words (5 x 1= 5)

 

16. Mention the contributions of Koch to the field of immunology.
17. What is a paratope?
18. Define zone of equivalence.
19. Define agglutination.
20. What are haemetopoietic stem cells?

 

 

PART  B

 

1. Answer any five of the following, each answer not exceeding 350 words ( 5 x 8= 40)

 

21. Mention the important milestones in immunology.
22. Write notes on antigens.
23. Discuss about national immunity schedule for newborn.
24. Give an account of radioimmunoassay.
25. Discuss briefly about immunoelectrophoresis.
26. Discuss briefly about T lymphocytes.
27. Explain about polyclonal antibodies and mention how are they produced?
28. Write notes on humoral immunity.

 

PART C

1. Answer the following, each answer not exceeding 1500 words.

(2 x 20 = 40)

29. Write detailed notes on antibodies, their properties and structure

(or)

1. Give a detailed account of vaccines and their types.

 

30. Explain the process involved in Hybridoma technology

(or)

1. Give a detailed account of Cell mediated immunity.

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

   B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

FIFTH SEMESTER – NOVEMBER 2010

    PB 5406 – IMMUNOLOGY AND IMMUNO TECHNOLOGY

 

 

 

Date : 11-11-10                     Dept. No.                                        Max. : 100 Marks

Time : 9:00 – 12:00

 

PART A (20 marks)

 

Answer all questions.

 

1. Choose the correct answer: (5 x 1 = 5)

 

1. Vaccine against rabies was first developed by
2. a) Koch b) Pasteur                    c) Jenner                     d) Kohler
3. Paratope is seen in the
4. a) antigen b) immunogen                        c) hapten                     d) antibody
5. Reaction of membrane bound antigen with labeled antibody is done in
6. a) Macini method b) RIA                          c) Immunoblotting      d) ELISA
7. Which of the following cells are involved in cytotoxicity?
8. a) Helper T cells b) Killer T cells                        c) Plasma cells                        d) Memory B cells
9. Hybridoma technology was first developed by
10. a) Kohler b) Mittelman               c) Yallow                     d) Prescott

 

 

1. State whether the following statements are true or false: (5 x 1 = 5)

 

6. Secretion of HCl in the stomach is an example for anatomical barrier.
7. All allergens are antigens.
8. Radial immunodiffusion involves radioactively labeled antibodies.
9. Naive B cells are those which have not been in contact with any antigens.
10. Myeloma cells are used in hybridoma technology because they are immortal.

 

 

III. Complete the following:                                                                                                                     (5 x 1  = 5 )

 

11. OPV and IPV are administered to confer immunity against _________.
12. The molecular weight of heavy chain is __________.
13. Reaction of insoluble antigen with soluble antibody is called ________.
14. B cells which can recognize antigens on subsequent entry are called ________..
15. HAT medium is used to culture ___________ .

 

1. Answer the following, each in about 50 words: (5 x 1= 5)

 

16. Mention any two contribution of Landsteiner.
17. List any two features of IgM.
18. Define haemagglutination.
19. Define plasma cells.
20. What is serum?

 

PART  B

 

Answer the following, each answer not exceeding 350 words. Draw diagrams wherever necessary:                                                                                                                                                                          ( 5 x 7= 35)

 

21. Give an account of the contribution of Pasteur and Koch.

(or)

1. Write short notes on immunization schedule.
2. Explain briefly about antigens and their properties.

(or)

1. Explain the structure of a typical antibody molecule.
2. Give an account of immunoelectrophoresis.

(or)

1. Write short notes on immunodiffusion.
2. Discuss briefly about cytokines and their importance.

(or)

1. Write short notes on T lymphocytes.
2. What are polyclonal antibodies? Explain the method involved in their production.

(or)

1. Explain the methods involved in the production of antiserum.

 

PART C

 

Answer any three of the following, each answer not exceeding 1200 words. Draw diagrams wherever necessary:                                                                                                                                                    (3 x 15= 45)

 

26. Describe in detail about innate and adaptive immunity.
27. Explain in detail about immunoglobulins, their classification and properties.
28. Discuss in detail about immunoblotting technique.
29. Explain in detail about the organs of immune system.
30. Describe the steps involved in the production of monoclonal antibodies.

 

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