LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

VE 22

SIXTH SEMESTER – April 2009

PB 6606 / 6601 – FERMENTATION TECHNOLOGY

 

 

 

Date & Time: 23/04/2009 / 9:00 – 12:00   Dept. No.                                                   Max. : 100 Marks

 

                                   PART –A                                                    (20 marks)

 

1. Choose the correct answer (5×1=5)

 

1. The industry which uses the principle of fermentation is

1. Food industry b. Paint industry c. Chemical industry   d. None

 

1. The microbes are preserved in small laboratories by

1. Daily Subculture b. Refrigeration c. Boiling        d. None

 

1. The antifoam agent used  in fermentor is

1. Soap b. Salt c. Lard oil    d. Vanaspati

 

1. In the fermenter aeration is done by

1. Sparger b. Agitators c. Impellors             d.  All

 

1. The salt used in precipitation of enzymes is

1. Ammonium sulphate b. Calcium sulphate c. Nickel sulphate   d. None

 

1. State whether the following statements are True or False (5×1=5)

 

1. Alcohol industry is the most profitable industry.

 

1. Parasexual cycle in yeast is useful for strain improvement.

 

1. Steam is the best sterilant for sterilisation of a fermenter.

 

1. Sonication cannot be used for cell disruption.

 

1. Biosensors are a boon to Fermentation industry.

 

III. Complete the following                                                                      (5×1=5)

 

1. ______________ is a fermented dairy product.

 

1. The sterilisation of heat sensitive substances is done by _________________.

 

1. Cryopreservation is carried out at ____________°C temperature.

 

1. The change in pH in media in the Fermentor is indicated by __________.

 

1. The mixing of components of media in a fermentor is done by the _________ .

 

 

 

 

IV Answer the following within 50 words                                       (5×1=5)

Write short notes on

 

1. Solvent extraction

 

1.  DO probe

 

1. Valves of fermentor

 

1. Fed batch cultures

 

1. Biotransformation

 

PART – B

Answer any FIVE of the following in about 350 words                       (5×8=40)

 

1. Write on range of Fermentation processes available for industry.
2. Explain Sterilization and its importance in fermentation.
3. Describe the body construction and basic functions of each part of fermentor.
4. Describe computerized control system available in fermentor
5. Explain the use of chromatography in down stream processing
6. Elaborate on the importance of effluent treatment after fermentation.
7. Describe the scope and importance of recombinant products in fermentation.
8. Precipitation is an important step in purification- Justify

 

PART-C

Answer the following in about 1500 words                                          (2X20=40)

 

29. a. Write in detail about the different types of fermentors.

OR

1. Describe the methods used in preservation of isolated cultures.

 

1. a. Explain the filtration and centrifugation in Bioprocess industry.

OR

1. Discuss in detail the Microbial growth kinetics and its use in fermentation .

 

 

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