LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.
FIFTH SEMESTER – APRIL 2012
PB 5406 – IMMUNOLOGY AND IMMUNOTECHNOLOGY
Date : 30-04-2012 Dept. No. Max. : 100 Marks
Time : 1:00 – 4:00
Part –A
Answer all, each answer within 50 words: (10×2=20 marks)
- What is agglutination?
- Define cell mediated immunity.
- Define Haptens.
- What is the function of suppressor T cells?
- Define epitope.
- How hybridomas are selected?
- Distinguish between cytokines and hormones.
- Define antigens.
- What are the two pathways involved in monoclonal antibodies?
- What are cytotoxic T cells?
Part B
Answer the following each answer within 500 words. Draw diagrams wherever necessary:
(5×7=35 marks)
- a) Explain innate and adaptive immunity.
OR
- b) How will you isolate serum from blood?
- a) Write short notes on:
- i) agglutination inhibition
- ii) complement fixation
OR
- b) Explain radioimmunoassay technique.
- a) Explain the properties of Cytokines.
OR
- b) Describe the steps involved in western blot.
- a) Explain T-lymphocytes.
OR
- b) Write about primary and secondary immune response?
- a) Differentiate polyclonal antibodies from monoclonal antibodies.
OR
- b) Explain the pathways involved in hybridoma technology.
Part C
Answer any three of the following each answer within 1200 words. Draw diagrams wherever necessary:
(3×15=45 marks)
- Explain the different types of immunoglobulins.
- Explain antigen-antibody interaction.
- Discuss the applications of monoclonal antibodies.
- Explain the types and role of cytokines in immunity and mention its functions.
- Define and explain cell mediated and humoral immunity.
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