LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

FIFTH SEMESTER – APRIL 2012

PB 5518/PB 5512/PB 5504 – PLANT BIOTECHNOLOGY

 

 

 

Date : 03-05-2012              Dept. No.                                        Max. : 100 Marks

Time : 9:00 – 12:00

 

                                                                   PART –A                                  

 

Answer ALL questions;  Each within 50 words only:                                    (10×2=20marks)

 

1. Define biotechnology.
2. What is Aseptic condition?
3. Define Caulogenesis.
4. What is a Fusogen?
5. What are nif genes?
6. What is T-DNA?
7. What are cloning vectors?
8. What are Microprojectiles?
9. Give the use of npt II gene.
10. What are cry genes?

     PART –B                                  

 

Answer the following each in 500 words only;

Draw diagrams and flow charts wherever necessary:                                    (5×7=35 marks)

 

11. a) Give a brief account on sterilization methods.

(OR)

1. b) Write short note on media composition.

 

12. a) Describe the procedure used for embryo culture.

(OR)

1. b) Give an account on somatic embryogenesis.

 

13. a) Explain the genetic organisation of mitochondrial DNA.

(OR)

1. b) Give a brief note on cytoplasmic male sterility.

 

14. a) Describe various cloning vectors.

(OR)

1. b)  Write short note on any one blotting technique.

 

15. a) Give the strategies used for the development of transgenic plants against herbicides.

(OR)

1. b) Write briefly on genome mapping.

 

 

 

 

   PART –C                         

 

Answer ANY THREE of the following, each within 1200 words only;       (3 x 15 = 45 marks)

Draw diagrams and flow charts wherever necessary:

 

16. Explain in detail on cell and suspension culture. Add a note on its applications.
17. Write a detailed account on meristem culture. Add a note on its importance in the field

of agriculture.

18. Write an essay on Agrobacterium mediated gene transformation.
19. Describe in detail on various direct gene delivery methods.
20. Give a detailed account on molecular markers.

 

 

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