LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.
FIFTH SEMESTER – NOVEMBER 2012
PB 5518/5512/5504 – PLANT BIOTECHNOLOGY
Date : 08/11/2012 Dept. No. Max. : 100 Marks
Time : 9:00 – 12:00
PART – A
ANSWER THE FOLLOWING, EACH WITHIN 50 WORDS: (10 x 2 = 20 marks)
- Define callus.
- What is an explant?
- What is a cybrid?
- Define vector.
- What are nod genes?
- Distinguish between intron and exons.
- Mention the role of Restriction endonuclease Type II.
- Define microinjection.
- Write notes on RAPD.
- What is Bt-gene?
PART – B
ANSWER THE FOLLOWING, EACH WITHIN 500 WORDS.
DRAW DIAGRAMS, FLOWCHARTS WHEREVER NECESSARY: (5 x 7 = 35 marks)
- a) Enumerate the importance of Plant tissue culture.
(OR)
- b) Give a brief account on the methods of sterilization.
- a) How are artificial seeds produced under in vitro condition?.
(OR)
- b) Write notes on cryopreservation.
- a) Describe the structure and organization of chloroplast DNA.
(OR)
- b) Write an account on the genetic organization of Ti plasmids.
- a) Briefly explain Polymerase Chain Reaction.
(OR)
- b) Write short notes on
(i) Cloning vectors
(ii) Genomic library
- a) Give an account on DNA finger printing.
(OR)
- b) Write notes on importance of molecular markers in genome mapping.
PART – C
ANSWER ANY THREE OF THE FOLLOWING, EACH WITHIN 1200 WORDS.
DRAW DIAGRAMS, FLOW CHARTS WHEREVER NECESSARY: (3 x 15 = 45 marks)
- Write an account on callus culture and mention its significance.
- Give an illustrated account of Isolation and fusion of protoplasts.
- Describe the molecular interaction of Rhizobium with leguminous plants.
- Write an essay on Agrobacterium mediated gene transfer.
- Describe the development of transgenic plants with reference to insect resistance.
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