LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
B.Sc. DEGREE EXAMINATION – PHYSICS, COMPUTER SCI. & APPL.
THIRD SEMESTER – APRIL 2012
PB 3208/3202 – BIOINFORMATICS – I
Date : 02-05-2012 Dept. No. Max. : 100 Marks
Time : 9:00 – 12:00
PART- A
Answer all the questions: (20 marks)
I Choose the correct answer : (5×1=5 marks)
- Where is ribosome located in plant and animal cell?
- a) inside the nucleus b) on the endoplasmic reticulum
- c) near the cell membrane d) inside the vacuole
- The database that classifies protein according to their structure and function is _______________.
- a) PIR-PSD b) i Proclass c) ALN d) RESID
- The one that collects and processes sequence data for PIR-international is ________________.
- a) MIPS b) PATS c) SMD d) DIP
- Cells were first discovered by _____________.
- a) Robert hooke b) George kohler c) Edward jenner d) Pasteur
- Identify the protein structure analysis database.
- a) RCSB-PDB b) RasMol c) ProtParam d) Web-Thermodyn
II State whether the following statements are True or False: (5×1=5 marks)
- ProtParam computes various physico-chemical properties of proteins.
- UAA is a start codon.
- 3ˊ- 5ˊstrand is called a non-coding strand.
- RasMol is licensed dually.
- Smith-Watermann algorithm is used to align multiple sequences.
III Complete the following: (5×1=5 marks)
- JSTOR stands for ________________.
- PDB is generated by ______________.
- Secondary structure of DNA was proposed by _________________.
- RasMol is otherwise called as ___________.
- Low helical stability region is predicted by _______________.
IV Answer the following each in about 50 words: (5×1=5 marks)
- What are restriction enzymes?
- Define protein synthesis.
- Name the 3 institutes that contribute towards International Nucleotide Sequence database
collaboration?
- Illustrate the central dogma of molecular biology.
- Write the applications of HGP.
PART – B
Answer the following, each answer within 500 words; Draw diagram wherever necessary: (5 × 8 = 40 marks)
- a) Give an account on the cytoplasmic organelles of eukaryotic cell.
OR
- b) Briefly explain transcription process.
- a) Write notes on i) EMBL ii) DDBJ
OR
- b) Give details of the currently used human genome project.
- a) Write short notes on i) disease database ii) journal database
OR
- b) Explain the steps involved in Needleman and Wunsch algorithm.
- a) Describe Chou- Fasman method.
OR
- b) Give an account on different types of BLAST program.
- a) Explain the software used for visualizing the 3D structure of proteins.
OR
- b) Elucidate the software used for predicting the physical properties of nucleic acids.
PART – C
Answer any TWO of the following, each answer within 1500 words; Draw diagram wherever necessary: (2 × 20 = 40 marks)
- Draw a Smith-Watermann algorithm for the following using the values of match: 1, mismatch:
0, gap: -1
ADCNGRQCLCRPM
AGCGNRCKCRP
- Describe the structure and functions of DNA.
- Write notes on i) PIR ii) Swissprot
- Explain secondary structure prediction on proteins.