LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.
FIFTH SEMESTER – April 2009
PB 5408 – BIOPHYSICS
Date & Time: 04/05/2009 / 1:00 – 4:00 Dept. No. Max. : 100 Marks
PART A (20 marks)
Answer All questions
I Choose the Correct Answer (5 x 1 = 5 marks)
- In industries ultrafiltration is used to separate ____________.
- biomolecules b. carbohydrates c. proteins d. all the above
- __________is the number of moles of a solute dissolved in a liter of solution.
- molality b. molarity c. normality d. PPM
03.___________ is used to visualizing DNA in UV light.
- agarose b. ethidium bromide c. SDS d. silver nitrate
- In Fluorescence microscope ____________ stain is used for lipid.
- cellofluor white b. Nile blue c. acridine orange d. none of the above
- __________ are the most dangerous form of radiation emitted by a nuclear explosion.
- alpha rays b. gamma rays c. beta rays d. x rays
II State whether the following statements are True or False (5 x 1 = 5 marks)
- Brownian movement may be used to distinguish between solutions and colloids.
- Hydrogen ion concentration is the number of moles of hydrogen ions per cubic
decimeter.
- Size exclusion chromatography is also called as affinity chromatography.
- Gases flow much easier than liquids or solids.
- Apoptosis is also called as programmed cell death.
III. Complete the following (5 x 1 = 5 marks)
- __________ in the air is an essential gas for life.
- pH is a measurement of the concentration of __________ ions in a solution.
- Agarose is a linear polymer extracted from ______________.
- The ability of the medium to reduce the light velocity is called ___________.
- Unnatural cell death is called as _______________.
- Answer all, each in about 50 words (5 x 1 = 5 marks)
- What is Brownian movement?
- Define Osmosis.
- What is Adsorption?
- Define refraction.
- Write notes on cell death.
PART B (5 x 8 = 40 marks)
Answer any five, each within 350 words only. Draw diagrams and flowcharts wherever necessary.
- Explain the important characteristics and theories of gases.
- Write detailed notes on percentage of concentration.
- Briefly describe the principles of Ion exchange chromatography and its uses.
- Write the principle, mechanism and uses of colorimetry.
- Emulsions and suspensions.
- Describe the following
- i) Wave nature of light.
- ii) Interference.
- Write notes on radioimmunoassay and its uses.
- Mention the biological effects and uses of radiation.
PART C (2 x 20 = 40 marks)
Answer the following, each within 1500 words only. Draw diagrams and flowcharts wherever necessary.
- Write notes on the followings
- i) Different types of chromatography.
- ii) 3 important theories of acids and bases.
iii) Immunofluoresence.
OR
- b) Elaborately describe the principle and uses of Gel electrophoresis.
- a) Write detailed notes on two types of electron microscope.
OR
- b) Write notes on the following
- i) Properties of colloids and their types with examples.
- ii) Affinity chromatography and its uses.
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