LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & BIOTECHNOLOGY

IB 10

FOURTH SEMESTER – APRIL 2006

                                                              PB 4504 – MICROBIOLOGY

 

 

Date & Time : 25-04-2006/9.00-12.00         Dept. No.                                                       Max. : 100 Marks

 

 

Part A

 

Answer all the questions                                                                     (20 marks)

 

I Choose the best answer                                                                                (5 x 1 = 5)

 

1. Pick out the scientist associated with phagocytosis.

1. Metchnikoff b. Ehrlich c. Lister           d. Miller

 

1. Statement 1: Lophotrichous condition refers to flagella present at both ends.

Statement 2: Salmonella exhibits peritrichous flagella.

1. 1. Statement 1 is correct, but statement 2 is incorrect.
   2. Statement 1 is incorrect, but statement 2 is correct
   3. Both statements are correct    d. Both statements are incorrect.

 

1. Which one of the following can be used in industrial production of proteases?

1. Aspergillus flavus b. Bacillus amyloliqifaeciens
2. E. coli d. Saccaromyces griseous

 

1. Non-cyclic photophosphorylation generates

1. ATP only b. NADPH₂ only c. ATP & NADPH₂    d. FADH

 

1. Lignin is degraded by ————- during organic matter decomposition.

1. Phycomyces b. Ascomycetes       c. Basidiomycetes       d. Deuteromycetes

 

II State whether the following statements are true or false.                           (5 x 1 = 5)

 

1. Hanging drop method helps to observe motility of bacteria.
2. SPC refers to Standard Plate Colony.
3. Most plant viruses have DNA.
4. Magnesium acts as cofactor in enzymic reaction.
5.  E. coli helps in bioflocculation.

 

III Complete the following.                                                                            (5 x 1 =5)

 

11. Kohler and Milstein were the first to produce ———— ————–.
12. ————– refers to rapid drying in a ————– state.
13. Uptake of naked DNA into bacterial cell is known as ————.
14. Fructose 1,6 diphosphate is cleaved into DHAP & glyceraldehydes 3 phosphate by an enzyme ————-.
15. Pork is rapidly spoiled due to its high content of ————-.

 

 

IV Answer the following in about 50 words each.                                        (5 x 1 =5)

 

16. Why Gram staining is called a differential staining?
17. Distinguish between streptomyces and streptococci.
18. What is mycophage?
19. Define merozygote.
20. What are aflatoxin?

 

Part – B

 

Answer any FIVE of the following, choosing not more than three in each section. Each answer not to exceed 350 words.                                     (5 x 8 = 40)

 

Section – A

21. Write down the salient aspects of Whittaker’s 5 kingdom concept.
22. Give the sequence of events which take place during the normal growth of bacteria.
23. In the form of a tabular column, bring out the various types of bacteriophages.
24. Expand the following with a footnote:
25. BGA b. PPLO          c. DMC           d. TMV

 

Section – B

25. Give an account of industrial production of proteases. Add a note on their applications.
26. Write notes on the role of microbes in genetic engineering.
27. Illustrate and explain sulfur cycle.
28. Describe the process of penicillin production.

 

Part – C

 

Answer the following, not exceeding 1500 words each. Draw diagram wherever necessary.                                                                                              (2 x 20 = 40)

 

30. a. Classify bacteria based on shape and arrangement of cells, nutrition, temperature and oxygen requirement.

Or

1. Give an account of viruses based on the general characteristics, capsid nature, host range and tissue affinities.

 

31. a. Give a detailed notes on bacterial conjugation.

Or

1. Explain sewage waste water treatment.

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc.

IB 37

DEGREE EXAMINATION –PLANT BIOLOGY & PLANT BIO-TECH.

FOURTH SEMESTER – APRIL 2007

PB 4504 – MICROBIOLOGY

 

 

Date & Time: 21/04/2007 / 9:00 – 12:00          Dept. No.                                                     Max. : 100 Marks

 

 

 

Part – A (20 marks)

 

Answer all the questions.

I  Choose the best answer                                                                               (5 x 1 = 5)

 

1. Statement A: Monerans include all unicellular eukaryotes.

Statement B: Whittaker coined the term “protists”.

1. 1. Statement A is correct, but statement B is incorrect.
   2. Statement B is correct, but statement A is incorrect.
   3. Both A and B are correct        d. Both A and B are incorrect

 

1. Match A Mesophiles B. Obligate anaerobes   C. Acid fast    D. Coccobacilli

1. E. coli ii. Closteridium            iii. Neisseria    iv. Mycobacterium
2. A – ii B – i C – iv              D – iii
3. A – ii B – i C – iv              D – iii
4. A – ii B – i    C – iv              D – iii
5. A – ii B – i C – iv              D – iii

 

1. Griffith used ———– for his experiment on transformation.

1. Streptococcus b. Staphylococcus c. Bacillus        d. E. coli

 

1. Which one of the following involved in denitrification process?

1. Nitrosomonas b. Nitrococcus c. Nitrospira    d. Corynebacterium

 

1. Catalytic sites of enzymes has got more of ————-.

1. Methionine b. Arginine c. Glycine        d. Glutamine

 

II  State whether the following statements true or false.                               (5 x 1 = 5)

 

6. Negative staining is used to stain gram-negative bacteria.
7. Fastidious organisms require specific growth requirements for their course.
8. Competancy attained during exponential phase.
9. Lytic phages are otherwise known as avirulant phages.
10. Rhizobium fixes atmospheric nitrogen.

 

III  Complete the following.                                                                           (5 x 1 = 5)

 

11. In —————— drop preparation ———— slide is used.
12. ———— and ———— are those which grow either in the presence or absence of

oxygen.

13. Microbes that requires specific nutrients for growth is termed as ————.
14. —————- are viruses which have algae as their hosts.
15. Toxin produced by Aspergillus flavus is ————–.

 

 

 

 

IV Answer the following each within 50 words.                                           (5 x 1 = 5)

 

16. Mention the contributions of each of the following scientists:
17. Lister b. Lederberg c. Ehrlich         d. Jenner
18. What are gas vacuoles? What is their function?
19. Differentiate coenzyme and cofactors.
20. Distinguish between icosahedral and helical viruses.
21. What is bioflocculation?

 

Part – B

 

Answer any FIVE of the following, each not exceeding 350 words.            Draw necessary diagrams.                                                                                                                              (5 x 8 = 40)

 

21. Tabulate the differences between prokaryotes and eukaryotes.
22. Classify bacteria based on shape and arrangement.
23. Write about the cultivation of viruses.
24. Expand the following with a foot note each.
25. PPLO b. Ag – ab       c. Taq              d. nm               e. TMV            e. CPE
26. Compare cyclic and non-cyclic photophosphorylation in phototrophs.
27. Briefly explain the mechanism of bacterial transformation.
28. Explain phosphorous cycle. Add note on the roles of VAM in agriculture.
29. Outline the protocol for industrial production of vinegar.

 

 

Part – C

 

Answer the following, each not exceeding 1500 words.                               (2 x 20 = 40)

 

29. (a) What are pure cultures? Discuss the methods of isolation of pure cultures.

Or

(b) Give an account of lytic cycle. How does it differ from lysogenic cycle?

 

 

30. (a) With the outline explain in detail about the pentose phosphate pathway.

Or

(b) Give an account on microbial food spoilage. Explain the methods of food

preservation.

 

 

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         LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

VE 11

FOURTH SEMESTER – April 2009

PB 4504 – MICROBIOLOGY

 

 

 

Date & Time: 24/04/2009 / 9:00 – 12:00  Dept. No.                                                Max. : 100 Marks

 

 

                                PART –A                                                    (20 marks)

 

1. Choose the correct answer (5×1=5)

 

1. The scientist Known as Father of Microbiology is

1. Louis Pasteur b. Alexander Fleming c. Robert Koch       d. Joseph Lister

 

1. The structure associated with conjugation by bacteria are

1. Pilus b. Flagella c. Mesosome         d. Cilia

 

1. The organisms which grow at 37 °C are called

1. Thermophiles b. Eosinophiles   c. Mesophiles   d. Psychrophiles

 

1. The bacteriphage capsid is usually made of

1. Lipid b. Protein c. Carbohydrate     d. Nucleic acid

 

1. The organism used in the lactic acid production is

1. Penicillium spp b. Aspergillus spp c. Lactobacillus spp   d. Bacillus spp

 

1. State whether the following statements are True or False (5×1=5)

 

1. Joseph Lister gave the world the first antiseptic – Carbolic acid .

 

1. Treponema palladium is a spiral thick and rigid.

 

1. Bacteriophages help in transduction.

 

1. Griffith experiment proves the process of conjugation.

 

1. Sewage pollution in drinking water is indicated by the presence of E.coli.

 

III. Complete the following                                                                      (5×1=5)

 

1. ____________  is an example of acid fast bacterium.

 

1. Log phase of growth is also known as ________________ phase.

 

1. _______________ pigment present in Sulpur bacteria  helps trap sunlight.

 

1. The expansion of BOD is ______________.

 

1. The viruses are cultivated using ______________.

 

 

 

 

IV Answer the following within 50 words                                       (5×1=5)

Write short notes on

 

1. Koch`s postulates

 

1.  Spread plate  technique

 

1. RUBISCO

 

1. Virion

 

1. VAM fungi

 

PART – B

Answer any FIVE of the following in about 350 words                       (5×8=40)

 

1. Write the principle and method of Gram Staining .
2. Explain ultra structure of bacteria.
3. Describe the light reactions of photosynthesis in bacteria.
4. Discuss whittakers  S Kingdom concept.
5. Explain the concept of lysogeny.
6. Elaborate on food spoilage caused by bacteria.
7. Describe the waste water treatment by aerobic means.
8. Write about the types of cuetue media based on physical state and chemical composition.

 

PART-C

Answer the following in about 1500 words                                          (2X20=40)

 

 

• Write in detail the process of recombination in bacteria.

OR

1. Describe different quantitative measurements used to Find bacterial growth.

 

30a. Explain the biogeochemical cycles present in bacteria.

OR

1. Discuss in detail the structure, types and classification of bacteriophages.

                                             

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

FOURTH SEMESTER – APRIL 2011

PB 4508/PB 4504/PB 3500 – MICROBIOLOGY

 

 

 

Date : 07-04-2011              Dept. No.                                        Max. : 100 Marks

Time : 1:00 – 4:00

 

PART-A (20 Marks)

Answer ALL  questions

 

1. Choose the correct answer                                                             (5X1=5 marks)

 

1. ______ method is used to stain Mycobacterium
2. Gram staining Acid-Fast staining    c. Simple staining   d.  Giemsa staining

 

2. Bacterial cells that exhibit a variety of shapes are called ______.
3. Pleomorphic Palisade   c. Trichomes   d. None of the above

 

3. When the viral genome can become integrated in to the bacterial genome, they are known as ______
4. Episome b. Prophage            c.Plasmids                    d.  None

 

4. The infection of a bacterium by the phage nucleic acid is known as _______
5. Transformation b. Conjugation           c. Transduction      d. recombination

 

5. Vinegar is otherwise called as _______
6. Citric acid b. Acetic acid  c. Lactic acid  d. Sulphuric acid

 

1. State whether the following statements are TRUE or FALSE         (5X1=5 marks)

 

6. Pure culture is otherwise known as axenic culture.
7. Facultative anaerobic bacteria are those which can grow without oxygen but

can utilize oxygen if it is present.

8. Generalized transduction provides a means for mapping bacterial genes.
9. Viruses infecting fungi are called cyanophages.
10. Rhizopus nigricans spoils bread

 

• Complete the following          (5X1=5 marks)

 

11. ______ introduced principle of aseptic surgery.
12. Culture medium that allows one to distinguish between or among different

microorganisms based on a difference in colony appearance is _______.

13. _______ is an agent that can carry a foreign DNA fragment into a host cell.

 

14. The zone of lysis produced by phages upon incubation on a lawn culture

of bacteria is known as _______.

15. Penicillin was discovered by ________.

 

1. Define the following                                                             (5X1=5 marks)

 

16. Monera
17. Lyophilization
18. Log Phase
19. Virion
20. BOD

PART-B

 

Answer the following each within 500 words.  Draw  diagrams wherever necessary

(5X7=35 marks)

 

21. a) List the contributions of:
22. Robert Koch (3.5 marks)
23. Louis Pasteur (3.5 marks)

(or)

 

1. b) Write the principle and procedure of Gram staining.

 

22. a) Describe the ultra structure of Bacterial cell.

 

(or)

 

1. b) Explain briefly any two methods of isolating pure culture.

 

23. a) Give an account on Enzymes: its nomenclature and characteristics.

 

(or)

1. b) Write notes on TCA cycle.

 

24. a) Briefly explain about the cultivation of viruses.

 

(or)

 

1. Give a brief account on classification of viruses.

 

25. a) Elaborate on the various practices used for food preservation.

 

(or)

 

1. b) Industrial production of Alcohol- Explain.

 

 

PART-C

 

Answer any THREE of the following, each with 1200 words

Draw  diagrams wherever necessary                                                                   (3X15=45 marks)

 

26. Discuss in detail about the major characteristics of microorganisms.
27. Describe in detail about the techniques used in the quantitative measurement

of bacterial growth.

28. Write in detail:
29. Conjugation (7.5 marks)
30. Transformation (7.5 marks)
31. Elaborate in detail about the structure and lytic cycle of Bacteriophages.
32. Give a detailed account on sewage treatment.

 

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

FOURTH SEMESTER – APRIL 2012

PB 4508/PB 4504 – MICROBIOLOGY

 

 

 

Date : 21-04-2012              Dept. No.                                        Max. : 100 Marks

Time : 1:00 – 4:00

 

PART-A

 

Answer the following, each within 50 words only:                                                (10 x 2 = 20 marks)

 

1. Write the Accomplishments of Anton Van Leeuwenhoek.
2. Define Tyndallization.
3. What is meant by Lophotrichous Flagella? Give example.
4. What are Chemotrophs?
5. Define Fermentation.
6. What is Transformation?
7. What are Viroids?
8. What is a Capsid?
9. List the uses of Vinegar
10. Define Rhizosphere.

 

PART-B

 

Answer the following within 500 words. Draw diagrams wherever necessary:           (5X7=35 marks)

 

1.  a) Give an account on Gram’s Staining.

(OR)

1. b) Explain the contributions of Louis Pasteur.

 

1.  a) Write short notes on Bacterial growth curve.

(OR)

1. b) Briefly explain the methods of pure culture isolation.

 

13. a) With a neat diagram, explain the process of Conjugation.

(OR)

1. b) Explain the steps involved in gene cloning.

 

14. a) Write notes on cultivation of viruses.

(OR)

1. b) Give an account on the general characteristics of viruses.

 

15. a) Explain briefly about the industrial production of Penicillin.

(OR)

1. b) Write notes on Sulphur Cycle.

 

 

PART-C

 

 

Answer any THREE of the following, each with 1200 words:

Draw  diagrams wherever necessary:                                                                     (3X15=45 marks)

 

 

16. Explain in detail about the major characteristics of microorganisms.
17. Give a detailed account on the methods to quantitatively measure the bacterial growth.
18. Describe in detail the process of Transduction.
19. With a neat diagram, explain the ultra structure of T even Bacteriophage. Also add a note

on the lytic cycle.

20. Write short notes on:
21. a) Aerobic sewage treatment processes. (10 marks)
22. b) Methods to preserve fermented food. (5 marks)

 

 

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