LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – CHEMISTRY, ZOOLOGY

IB 7

FOURTH SEMESTER – APRIL 2006

                                                PB 4202 – MICROBIAL BIOTECHNOLOGY

 

 

Date & Time : 22-04-2006/9.00-12.00         Dept. No.                                                       Max. : 100 Marks

 

 

Part A

Answer all the questions                                                                         (20 marks)

1. Choose the correct answer (5 x 1 = 5 )

1. ________ are enzymes which acts on proteins.

1. a) amylases b) pectinases             c) proteases     d) lipases

1. The viral surface antigen of the hepatitis B virus is .

1. a) HbsAg b) HbcAg c)  HbeAg       d)  HbdAg

1. Malt extract contains ………… % of nitrogen.

4. a) 5 b) 5.5               c) 8.5               d)  80

1. The production of malt beverages is known as …….

1. a) Malting       b) Mashing            c) Brewing      d) Ripening

1. Which of the following one is an exopolysaccharide?

1. a) Xanthan            b) Inulin          c) Renin           d) Pectin

 

1. State whether the following statements are True or False (5 x 1  = 5 )

1. An endocellular enzyme is not secreted in the fermentation medium.
2. Immunizing agents that stimulate immunity to toxins are called toxoids.
3. Microorganisms can be preserved by Lyophilization.
4. Spirulina contains carotene pigments.
5. Biosensor is used for the quantitative estimation of important substances in body fluid

III. Complete the following                                                                              (5 x 1 = 5)

1. ______ is the first discovered antibiotic.
2. _______ are used in removal of starch sizing from woven cloth.
3. The technology used for the cultivation of eukaryotic cells in large scale is called as __________
4. The moisture content of soft cheese is  ________
5. VAM  refers to ________    __________   _________.

1. Answer the following in one or two sentences. (5 x 1 = 5)

1. What is a constitutive enzyme ?.
2. What is acquired immunity ?.
3. What is Beerwort?
4. Define transgenic microbe
5. What is single cell protein?

Part B

Answer any five of the following each in about 350 words. Draw necessary diagrams.                                                                                         (5 x 8 = 40)

1. Explain the microbial production of pectinase.
2. List out any 5 applications of microbial enzymes.
3. Explain the transformation of steroids.
4. Mention the different sources of Nitrogen and Carbon energy in fermentation industry.
5. Describe the methodology of wine production. Add a note on types of wine.
6. Define Bioplastics. Write notes on  Xanthan production and its applications.
7. With the flow chart, explain the steps involved in cheese production.
8. What is biosensor? Explain its working principle and its application.

Part C

Answer the following  questions each within 1500 words. Draw necessary diagrams

(2 x 20 = 40)

29. a) i) Describe the different stages of fermentation process (10 marks)
30. ii) Describe in detail the cultivation of mushroom. Add a note on their nutritional  composition (10 marks).

OR

1. b) i) Define Biofertilizer. Explain the method of mass cultivation of Azolla and Rhizobium. Add a note on their significance (12 marks).
2. ii) Define biopesticide. Why Bacillus thuringiensis is called as biopesticide?    (8 marks)
3. a) What are antibiotics ?. What are its properties ?. Give an account on the industrial production of penicillin.

OR

1. b) Give an account on the applications of aminoacids and explain the industrial production of L glutamic acid.

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – CHEMISTRY / ADV. ZOO. & BIO. TECH.

IB 39

FOURTH SEMESTER – APRIL 2007

PB 4202 – MICROBIAL BIOTECHNOLOGY

 

 

Date & Time: 25/04/2007 / 9:00 – 12:00              Dept. No.                                                         Max. : 100 Marks

 

 

 

 

Part A                                           (20 marks)

Answer All questions

 

I Choose the Correct Answer                                                                     ( 5 x 1 = 5 marks)

 

1. Technique of attachment of cells or protoplasts or enzymes to a matrix is known as
2. entrapment                                      b. immobilization
3. impregnation d. covalent bonding
4. Which of the following process is very important for the development of

characteristic flavor and aroma of wine?

1. Brewing                   b. Malting        c. Ageing                     d. Mashing
2. Most of the Vitamin B12 fermentation processes use ______ as the carbon source.
3. glucose b. sucrose        c. trehalose                  d. starch
4. Industrial production of enzyme is mostly carried out by ______ fermentation.
5. Submerged b. solid surface            c. liquid surface           d. both b and c
6. Entirely decomposed organic material refers to _______
7. fertilizer b. manure                    c. compost                   d. humus

 

II State whether the following statements are True or False                   (5 x 1 = 5 marks)

 

6. Phosphate solubilizing microorganisms are called as biofertilizers.
7. Yoghurt is a product of fermented milk.
8. Inhibitory relationships between microorganisms are called amensalism.
9. Maximum amount of citric acid is produced in Airlift or Stirred bioreactor.
10. Xanthan is an exopolysaccharide.

 

.

III. Complete the following                                                                     ( 5 x 1 = 5 marks)

 

11. ______involves transfer of genes between unrelated strains which are producers of

known substrates.

12. _______ refers to the microbial cells or total protein extracted from pure microbial

cell culture.

13. ______ results in change in a chemical molecule by means of microbial or

enzymatic reaction.

14. In food industry, Glutamic acid is used in the form of __________
15. ______ is a technique of seed-dressing with bacteria as water suspension

 

 

 

 

 

 

1. Answer all, each in about 50 words ( 5 x 1 = 5 marks)

 

16. What are Mycobeads?
17. Mention any two substrates used for oyster mushroom cultivation.
18. What is Algalization?
19. What is vinegar?
20. Mention any one microbe which produces polyhydroxyalkanoates.

 

PART B                       ( 5 x 8 = 40 marks)

Answer any five, each within 350 words only. Draw diagrams and flowcharts wherever necessary.

 

21. Explain the various substrates used for industrial fermentation.
22. Explain the fermentation process of beer production.
23. Write notes on the production of insulin
24. Write in brief for the production of Vitamin B₁₂
25. Mention any two microorganisms that are involved in the production of protease

enzyme, glutamic acid, acetic acid and citric acid.

26. Briefly explain the technology of citric acid production.
27. Write down the procedure of transformation of steroids.
28. Enumerate the significances of Xanthan, Bioplastics and Biosensor.

PART C                       ( 2 x 20 = 40 marks)

 

Answer the following, each within 1500 words only. Draw diagrams and flowcharts wherever necessary.

 

29a. i) With an example, explain how microbes play an important role in the production

of  dairy products.

 

ii). With the flow chart explain the methodology of mushroom cultivation. Write

down the procedure for  commercial preparation of mushroom.

 

(OR)

1. Give the outline of the entire fermentation process. Add a note on product recovery

processes (Down Stream processing)

 

30a. Write an essay on Biofertilizers.

(OR)

1. i) Explain the microbial technology of α – amylase production.

 

1. ii) Explain the working principle of modern device to quantify the

biomolecules in   our system. Add a note on its significance in other fields.

 

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